The bovine project was fortunate to have many sets of markers from different sources available to place the assembly on chromosomes. A challenge in using these was the difficulty in merging the multiple marker sets into a single consistent map. New software (Atlas) assembly components were developed to solve the conflicts in the merged marker sets and maximize their usage for scaffold placement and correction.
The majority of the sequence in the project is from the female animal, the genome sequence is described for the 29 autosomes and the X chromosome. However, as the BAC library was prepared from a male animal, and the BAC fingerprint contigs were built from random clones from that library, both the X and Y chromosomes are represented in the BAC fingerprint contigs. Representative BACs in all of the BAC fingerprint contigs were sequenced to low coverage, including Y chromosome BACs. Since the clone coverage on the sex chromosomes in the BAC library is half that of the autosomes, there will be less depth of clone coverage on the sex chromosomes and this may result in more gaps in the coverage of the sex chromosomes by BAC clones. The WGS sequence was from the female animal, so there is not additional WGS sequence to assemble with the low coverage BAC skim sequences for the Y chromosome, unless it is pseudoautosomal sequence from the X chromosome or autosomal sequence that is similar to the Y sequence. Since the BAC fingerprint contigs were used to build the combined BAC+WGS assemblies, there are genome sequence scaffolds from both sex chromosomes as well as the autosomes. The Y chromosome scaffolds are unlabeled in the unplaced chromosome.
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The order of the scaffolds on the chromosomes was refined based on the information from three sources: the fingerprint contig map (FPC)[21], human and dog synteny, and links by sheep BAC clones[13]. When any three adjacent scaffolds had order information from at least two of the three sources and the order was consistent among these sources but in conflict with the ILTX map[12], the order of the scaffolds was modified from the ILTX map order[12]. The scaffolds that were not oriented by the ILTX map[12] were oriented using the FPC information when such information was available.
YL produced the final assembly, developed methods for using different BAC assembly methods and combining the BAC and WGS assemblies. XQ produced the whole genome shotgun assemblies and performed mapping of the markers to these assemblies. XHS performed the synteny mapping to other mammalian genomes. HJ performed the BAC assemblies of pooled BACs and eBACs. YS modified methods he developed for sea urchin dealing with pooled BACs and merging BACs so that they could be used in the bovine project. KJD modified his code for merging BACs, advised on deconvolution of pooled BACs. SL, MS and MPK contributed mapping information and examined linkage data for all autosomes to quality check the assembly. YR provided read wrangling support by collecting sequence data and building the reads database prior to assembly. LZ evaluated paired-end data to quality check the assemblies. ES managed the BAC and pooled BAC processing and consulted on the use of that data. PH adjusted the software for the BAC-fishing assemblies and advised on the deconvolution of pooled BACs. KCW directed the genome assembly group and provided guidance and coordination, contributed to writing the manuscript. GW, co-director of the HGSC during this project, provided direction and coordination with the bovine community. RAG director of the HGSC, secured funding and provided project coordination and direction.
Fingerprint sensor modules, like the one in the following figure, made fingerprint recognition more accessible and easy to add to your projects. This means that is is super easy to make fingerprint collection, registration, comparison and search.
These modules come with FLASH memory to store the fingerprints and work with any microcontroller or system with TTL serial. These modules can be added to security systems, door locks, time attendance systems, and much more.
Prices for this sensor greatly vary from $10 to $50. We recommend checking the Fingerprint sensor module on Maker Advisor that compares the price in different stores. The fingerprint sensor modules featured on Maker Advisor should be compatible with this guide.
The fingerprint sensor module used in this project came with really thin wires, so soldering breadboard-friendly wires was needed. We recommend using different colors according to the pin function. In our case:
///! Helper class to communicate with and keep state for fingerprint sensorsclass Adafruit_Fingerprint { public:#if defined (__ AVR__) defined (ESP8266) defined (FREEDOM_E300_HIFIVE1) Adafruit_Fingerprint (SoftwareSerial * ss, uint32_t password = 0);#endif Adafruit_Fingerprint (HardwareSerial * hs, uint32_t password = 0);
Sure. But as Santos mentioned by using default library, you can only store it in Flash memory.However, if DownChar/UpChar commands are used, the templates in Sensor Buffers (CharBuffer1 or CharBuffer2) can be downloaded/uploaded to an external computer. And those templates can be utilized to be transfered to the other sensors connected to the other arduinos.This method can handle the process for an environment using multiple fingerprint sensors in different locations, and the data upload/download can be done over network using a ethernet module.
So basically this code is useful when we set up the fingerprint sensor, if I understand well.So it is not like the sketch to run when the arduino is already set up and disconnected from the laptop, is it ?
The second result concerns the transcriptomic fingerprint of EE. The availability of a horse-specific microarray and of the horse genome sequence has opened up the opportunities for further research into the biological impact of EE on horses. By coupling gene expression data with several bioinformatic analyses, we can show that EE and control conditions have divergent effects on gene expression in blood cells. According to the TELiS analyses, there is pronounced activation of CREB/ATF and GATA families of transcription factors in the control group. In the EE-treated horses, there was a tendency for upregulation of target gens of NF-κB (NF-κB and REL factors), MZF, and AP1. Overrepresentation of the CREB transcription factors in chronically isolated versus socially integrated people has been observed previously [45]. In another study, β-adrenergic activation of the GATA1 transcription factor was found in mice subjected to a social threat; a similar finding was reported for human depressive subjects (compared to control) [46]. Because CREB/ATF transcription factors convey adrenergic signals to the transcriptome in blood cells, it is possible that the activation of CREB/ATF and GATA transcription factors in the control group of horses might reflect the poor social interactions associated with the low learning performance and high levels of fearfulness in these horses. According to the oPOSSUM analysis, overrepresentation of transcription factors other than CREB/ATF and GATA was detected in the control horses; only the NF-κB-related findings were common between the two types of analysis. In human social genomic studies, IRF-1 is usually found to be downregulated in isolated/stressed individuals, and this phenomenon is thought to reflect an adaptive reaction to the low risk of dissemination of a viral infection in isolated individuals [47]. Here, this hypothesis is not supported by our data because control animals are isolated most of the time but display activation of IRF-1. Rather, activation of IRF-1 in the control group supports the notion that interferon induces a loss of appetite and sickness-like behavior because 4 out of 9 animals in the control group lost their appetite at the end of the experiment. A vet evaluated the animals with eating problems for infectious disease using standard clinical metrics (mostly core body temperature) and found no evidence of an active infection that might explain the observed transcriptomic alterations in immune cells. The oPOSSUM analysis showed overrepresentation of such factors as ARID3, NKX3-1, Foxq1, and FoxD3 (in addition to IRF-1) which are all involved in cell cycle regulation. Finally, IPA revealed that in the control group, a network of genes is upregulated that is involved in mismatch repair and cell death-related processes.
Pet businesses come to Gingr for a user-friendly experience. Gingr has the most advanced customization, automation, and communication tools of any pet business software. We also deliver the highest uptime and fastest performance in pet-care software, so you can rely on your software when you need it most.
Yes, the new version of Badge Studio is compatible with the documents generated with the previous Badge Studio version.From the Home Page, click on File> Open a document.Browse a document that was created with a previous version of Badge Studio software (.card file).Once the document is opened in Badge Studio, you may edit it and save it as wished.
From the USB drive provided by Evolis, double-click on Setup_BadgySoftware.exe or Setup_BadgySoftware.pkg to launch the installation. Follow the instructions of the wizard.You may also download the file from www.badgy.com and launch the installation on your computer.The setup will install the driver and the software. For this reason, it is necessary to wait for the full installation of the setup before plugging in the USB cable on your computer.
From the Badge Creation wizard that is displayed at the launch of the software, click on Get More Templates to have access to Badgy card template library or click here.Then you can select the badge templates by applications, sectors and colours.
There are two ways to upgrade to the PLUS edition.In the first case, you own a Badgy200: connecting the printer to your computer and launching the software automatically upgrades to the PLUS edition.In the second case, you own a Badgy100, you have a license activation code and an Internet connection.Check that your license has been activated by clicking on File>Badge Studio License: the edition version is displayed. 2ff7e9595c
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